Journal: Biomolecules

Article Title: Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation.

doi: 10.3390/biom9110711

Figure Lengend Snippet: Figure 4. FO activates osteoblast-specific protein expression and mineralization/calcification in osteosarcoma MG-63 human osteoblast-like cells. (A) MG-63 cells (3 × 103 cells/mL) were seeded overnight and then treated with FO (50 µg/mL and 100 µg/mL) or DEX (100 nM) for 7 days. Protein was extracted and western blotting analysis was performed using each specific antibody. β-Actin was used as the internal control of protein expression. All protein expression was normalized by the density of β-actin. The nuclear localization of runt-related transcription factor 2 RUNX2 (B) and osterix OSX (C) was measured by immunostaining. (D) ALP activity (mineralization) was measured using a TRACP & ALP assay kit. (E) In vitro calcification was detected by alizarin red staining. Significant differences among the groups were determined using the one-way ANOVA followed by Bonferroni correction. All data are presented as mean ± SEM (*** p < 0.001, ** p < 0.01, and * p < 0.05 versus untreated group). FO; fermented extract of C. gigas and DEX; dexamethasone. FO; fermented extract of C. gigas and DEX; dexamethasone.

Article Snippet: Specific antibodies against RUNX2, ALP, Col1α1, OCN, OSX, BMP4, β-actin, β-catenin, and nucleolin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, Control, Immunostaining, Activity Assay, ALP Assay, In Vitro, Staining

Journal: Scientific Reports

Article Title: Giant rotating magnetocaloric effect induced by highly texturing in polycrystalline DyNiSi compound

doi: 10.1038/srep11929

Figure Lengend Snippet: The temperature dependence of Δ S for different magnetic field changes along parallel ( a ) and perpendicular ( b ) directions, respectively. ( c ) The difference of Δ S between parallel and perpendicular directions as a function of temperature and magnetic field change.

Article Snippet: A small negative −Δ S value (inverse MCE) is observed below T N at 10 kOe along parallel direction because of the presence of AFM state, while it becomes positive with the increase of magnetic field.

Techniques:

Journal: Scientific Reports

Article Title: Giant rotating magnetocaloric effect induced by highly texturing in polycrystalline DyNiSi compound

doi: 10.1038/srep11929

Figure Lengend Snippet: Isothermal magnetization curves at 8 K ( a ) and 9 K ( b ) under applied fields up to 20 kOe by rotating the sample from perpendicular (90°) to parallel (0°) direction with a step of 10°. ( c ) The schematic describes the rotation of sample from perpendicular (90°) to parallel (0°) direction in magnetic field. ( d ) The Δ S R ( θ ) as a function of rotation angle at 8.5 K for different magnetic field changes.

Article Snippet: A small negative −Δ S value (inverse MCE) is observed below T N at 10 kOe along parallel direction because of the presence of AFM state, while it becomes positive with the increase of magnetic field.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects

doi: 10.3389/fbioe.2023.1098172

Figure Lengend Snippet: Effects of EVs on the osteogenic differentiation of rBMSCs (A) The observation of ALP and alizarin red staining (B) Quantification of ALP activity (C) Quantification of alizarin red staining (D) The expression of osteogenic genes (ALP, OCN and RUNX2) (E) The expression of osteogenic proteins (ALP, OCN and RUNX2). a, p < 0.05 compared with the control group; b, p < 0.05 compared with the 25 μg/ml EVs group; c, p < 0.05 compared with the 50 μg/ml EVs group.

Article Snippet: Western blotting was performed to detect the expression of osteogenesis-related proteins ALP (No. 60294-1-Ig, Proteintech), OCN (GTX64348, GeneTex) and RUNX2 (No. 20700-1-AP, Proteintech).

Techniques: Staining, Activity Assay, Expressing

Journal: International Journal of Molecular Medicine

Article Title: Baicalin increases hair follicle development by increasing canonical Wnt/β-catenin signaling and activating dermal papillar cells in mice

doi: 10.3892/ijmm.2018.3391

Figure Lengend Snippet: Baicalin upregulates the activity of dermal papillar cells in reconstituted mouse skin tissue. Levels of ALP (A) mRNA and (B) protein in reconstituted mouse skin tissue as measured using reverse transcription polymerase chain reaction and western blotting, respectively. Western blot band intensities were quantified using densitometry and ALP levels normalized to β-actin levels. (C) Representative western blots of ALP and β-actin in mouse skin tissue. (D) Histochemical analysis of ALP activity in hair follicles control and B100 groups was performed using a BCIP/NBT kit (magnification, ×40). Arrows indicate the ALP-positive cells. Control cells were treated with the vehicle only. * P<0.05 and ** P<0.01. ALP, alkaline phosphatase; Minoxidil, 100 μ mol Minoxidil treatment; B50, 50 μ mol baicalin treatment; B100, 100 μ mol baicalin treatment; B+IWR-1, 100 μ mol baicalin and 1 μ mol IWR-1 treatment.

Article Snippet: After blocking with 5% nonfat dry milk in TBST for 2 h at room temperature, the membrane was incubated with primary antibodies against the following: β-actin (BM0627; 1:200), lamin (BA1228; 1:200), Wnt3a (BA2628-2; 1:300), Wnt5a (155184-1-AP; 1:1,000) frizzled 7 (16974-1-AP; 1:2,000), disheveled 2 (12037-1-AP; 1:2,000), GSK3β 1 (22104-1-AP; 1:2,000), β-catenin (51067-2-AP; 1:5,000) LEF1 (14972-1-AP; 1:500) and ALP (11400-1-AP; 1:1,000; all Wuhan Proteintech Co., Ltd., Wuhan, China) overnight at 4°C.

Techniques: Activity Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Control

Journal: BMC Cell Biology

Article Title: Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

doi: 10.1186/s12860-017-0133-0

Figure Lengend Snippet: Mechanical strain impaired gene expression of certain cementogenesis-related biomarkers. a - f The relative mRNA levels of BSP, ALP, OCN, OPN, Runx2 and Osx in OCCM-30 cells in the presence or absence of 18 h mechanical strain. Total RNA was subjected to qPCR. Relative mRNA expression levels were normalized to GAPDH as an endogenous reference. The experiments were repeated 3 times. Bars represent means ± SD. * p < 0.05

Article Snippet: For immunoblotting, anti-PTHR1 antibody (Abcam, Cambridge, UK), anti-Runx2 antibody (Abcam, Cambridge, UK), anti-Osx antibody (Abcam, Cambridge, UK), anti-COL1 antibody (Abcam, Cambridge, UK), anti-OPN antibody (Abcam, Cambridge, UK), anti-ALP antibody (Biorbyt, Cambridge, UK), anti-OCN antibody (Biorbyt, Cambridge, UK), and anti-BSP antibody (Biorbyt, Cambridge, UK) were used at 1:1000 dilution.

Techniques: Gene Expression, Expressing

Journal: BMC Cell Biology

Article Title: Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

doi: 10.1186/s12860-017-0133-0

Figure Lengend Snippet: Intermittent PTH attenuated cementogenesis-related gene expression inhibition that was induced by mechanical strain. a Western blot analysis of BSP, ALP, OCN, COL1, Runx2 and Osx levels in cells of the control group, the strain group and the strain + PTH group that was treated with mechanical strain and intermittent PTH (also shown in Additional file : Figure S3). Except the control group, other two groups were both treated with 18 h mechanical strain, while the control group was placed in the bending box without mechanical strain. Then, the control group and the strain group were exposed to 3 cycles of vehicle treatment. Each cycle comprises 6 h vehicle medium and 18 h fresh medium. Meanwhile, the strain + PTH group were exposed to 3 cycles of intermittent PTH administration. Each cycle comprises 6 h PTH (50 ng/ml) medium and 18 h fresh medium. Results were representative of 3 experiments. b - g Densitometry of bands was performed and normalized to GAPDH. The control group was set to 1. Bars represent means ± SD of triplicate measurements. * p < 0.05

Article Snippet: For immunoblotting, anti-PTHR1 antibody (Abcam, Cambridge, UK), anti-Runx2 antibody (Abcam, Cambridge, UK), anti-Osx antibody (Abcam, Cambridge, UK), anti-COL1 antibody (Abcam, Cambridge, UK), anti-OPN antibody (Abcam, Cambridge, UK), anti-ALP antibody (Biorbyt, Cambridge, UK), anti-OCN antibody (Biorbyt, Cambridge, UK), and anti-BSP antibody (Biorbyt, Cambridge, UK) were used at 1:1000 dilution.

Techniques: Gene Expression, Inhibition, Western Blot, Control

Journal: BMC Cell Biology

Article Title: Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts

doi: 10.1186/s12860-017-0133-0

Figure Lengend Snippet: Sequences of primers used in qPCR

Article Snippet: For immunoblotting, anti-PTHR1 antibody (Abcam, Cambridge, UK), anti-Runx2 antibody (Abcam, Cambridge, UK), anti-Osx antibody (Abcam, Cambridge, UK), anti-COL1 antibody (Abcam, Cambridge, UK), anti-OPN antibody (Abcam, Cambridge, UK), anti-ALP antibody (Biorbyt, Cambridge, UK), anti-OCN antibody (Biorbyt, Cambridge, UK), and anti-BSP antibody (Biorbyt, Cambridge, UK) were used at 1:1000 dilution.

Techniques: Sequencing